ABSTRACT
The metal binding capacity of cysteine with three different metals Nickel, Copper and Lead was studied using UV-Vis spectrophotometer for which absorbance values were taken after interaction of cysteine with metal salt solutions (10ppm and 100ppm). Before taking above absorbance dilution factor was set using cysteine stock. The increase in peak intensity was observed when metal salt solution and metal saltcysteine solution were compared. Based on peak shift and peak intensity finally it can be concluded that the binding capacity of cysteine with Nickel is more, followed by lead and copper. The normal chromophore activity in cysteine is due to the sulphur in which the transition takes place from non bonding orbital’s to the excited antibonding orbital in the range of 210-215nm range. The binding of the metals with cysteine may affect the chromophore activity and may also lead to structural damage of the chromophore. This can give the decrease in the peak intensity or the complete shift in the peak. These results suggest that cysteine metal binding ability can be used for the removal of the metals in water purification. Also this property can be used in removal of metals from our body considering the fact that cysteine may not show adverse effect in the system. So we can go for designing a new type of drug containing cysteine which helps to prevent the accumulation of such metals and thus prevent us from adverse effect.